Abstract: In order to explore the effects of farmland management measures such as pesticide spraying and irrigation on zooplankton diversity in the ditch-wetland system of paddy field, community ecology methods were used to conduct a comparative study on zooplankton communities in the ditch-wetland system of paddy field under two farmland management modes, artificial management mode and traditional management mode. The results showed that: ① Different farmland management measures had no significant effect on zooplankton species in ditch-wetland. Rotiroids and copepods were both dominant species in water bodies, while cyclops latifolia and copepods nodal larval species were stable and dominant species in each water body. ② The artificial management model significantly increased the abundance of zooplankton in ditch-wetland, decreased the number of dominant zooplankton species in ditch-wetland, and decreased the Margalef species richness index D_MG and Shannon-Wienner diversity index H′. ③ Pesticide application in paddy field reduced the number and abundance of zooplankton species in ditch-wetland, and significantly decreased the species number, abundance and dominance of dominant rotifers. The D_MG of zooplankton in the drainage ditch and the D_MG and H′ of zooplankton in the wetland were significantly reduced. It can be seen that pesticide application in paddy field has a profound effect on zooplankton toxicity. The research results can provide a reference for effective farmland management measures such as pesticides, irrigation and ecological irrigation district construction.